CST, 28530S, Claudin-2 (E1H9O) Rabbit Monoclonal Antibody (IHC Formulated)

Monoclonal Antibody for studying Claudin-2. Validated for Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Claudin-2 (E1H9O) Rabbit Monoclonal Antibody (IHC Formulated) (CST #28530) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity Claudin-2 (E1H9O) Rabbit Monoclonal Antibody (IHC Formulated) recognizes endogenous levels of total claudin-2 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp196 of human claudin-2 protein. Background Tight junctions, or zonula occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces. Tight junctions are composed of claudin and occludin proteins, which join the junctions to the cytoskeleton (1,2). The claudin family is composed of 23 integral membrane proteins, and their expression, which varies among tissue types, may determine both the strength and properties of the epithelial barrier. Alteration in claudin protein expression pattern is associated with several types of cancer (2,3). Claudin-1 is expressed primarily in keratinocytes (4) and normal mammary epithelial cells, but is absent or reduced in breast carcinomas and breast cancer cell lines (5,6). Claudin-2 is expressed primarily in the proximal tubule of the normal mammalian kidney, where it regulates transepithelial ion (e.g., Na , Cl ) reabsorption (7). Increased expression of claudin-2 has been reported in some cancer cell lines (8), including A549 lung adenocarcinoma cells, where its nuclear distribution was positively associated with enhanced proliferation (9). Alternate Names claudin 2; Claudin-2; claudin2; CLD2; CLDN2; SP82 Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG