CST, 28559S, SCD1 (C12H5) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying SCD. Validated for Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, SCD1 (C12H5) Rabbit Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #28559) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity SCD1 (C12H5) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu35 of mouse SCD1 protein. Background Stearoyl-CoA desaturase 1 (SCD1) is a key lipogenic enzyme found in the endoplasmic reticulum that catalyzes the conversion of palmitoyl-CoA and stearoyl-CoA to palmitoleoyl-CoA (16:1) and oleoyl-CoA (18:1) (1-3). Palmitoleate and oleate are the major components of triglycerides, membrane phospholipids, and cholesterol esters (1). SCD1-knockout mice show improved insulin sensitivity and reduced body fat (1). Disruption of SCD1 in mouse brown adipose tissue strengthens insulin signaling and results in increased translocation of Glut4 to the plasma membrane and enhanced uptake of glucose (4). Furthermore, SCD1 is essential for the onset of diet-induced body weight gain (1) and insulin resistance in the liver (5). Alternate Names ACOD; Acyl-CoA desaturase; Delta-9 desaturase; delta-9-desaturase; Delta(9)-desaturase; FADS5; Fatty acid desaturase; hSCD1; MSTP008; predicted protein of HQ0998; SCD; SCD1; SCDOS; Stearoyl-CoA desaturase; stearoyl-CoA desaturase (delta-9-desaturase); stearoyl-CoA desaturase opposite strand Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG