CST, 2912S, GFP (D5.1) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying GFP jellyfish. Validated for Western Blotting. Highly specific and rigorously validated in-house, GFP (D5.1) Rabbit Monoclonal Antibody (Biotinylated) (CST #2912) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GFP (D5.1) Rabbit Monoclonal Antibody (Biotinylated) detects transfected levels of GFP or GFP-tagged proteins. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of GFP. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization (1). Alternate Names GFP; Green fluorescent protein Specification REACTIVITY: All SENSITIVITY: Transfected Only MW (kDa): 27 Source/Isotype: Rabbit IgG