CST, 2956S, GFP (D5.1) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GFP jellyfish. Validated for Western Blotting,Simple Western™,Immunohistochemistry (Paraffin). Available in 3 sizes. Highly specific and rigorously validated in-house, GFP (D5.1) Rabbit Monoclonal Antibody (CST #2956) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 34664 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity GFP (D5.1) Rabbit Monoclonal Antibody detects GFP, YFP, and CFP-tagged proteins exogenously expressed in cells. This antibody does not detect RFP-tagged proteins. Please note that the GFP, YFP, and CFP tags add approximately 27 kDa to the molecular weight of the fusion protein. Non-specific staining was observed in mouse brain by immunohistochemistry. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of GFP. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization (1). Alternate Names GFP; Green fluorescent protein Specification REACTIVITY: All SENSITIVITY: Transfected Only MW (kDa): 27 Source/Isotype: Rabbit IgG