CST, 2981S, Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody

Polyclonal Antibody for studying . Validated for Western Blotting,Immunoprecipitation,Peptide ELISA (DELFIA). Highly specific and rigorously validated in-house, Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody (CST #2981) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Peptide ELISA (DELFIA): 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody detects endogenous levels of proteins containing the Arg-X-Tyr/Phe-X-pSer motif. This antibody does not cross-react with nonphosphorylated serine or phospho-threonine proteins with the same motif or other phospho-serine/threonine-containing proteins and peptides without this motif. Species Reactivity: All Species Expected Source / Purification Polyclonal antibodies are produced by immunizing animals with Arg-X-Tyr/Phe-X-pSer-containing peptides . Antibodies are purified by protein A and peptide affinity chromatography. Background The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control (1-5). Binding of 14-3-3 is mediated through phospho-serine-containing proteins (6). Two different phospho-serine containing motifs are found using a degenerate phospho-serine-oriented peptide library technique, RSXS*XP and RXY/FXS*XP (6). Motif 2 (RXY/FXS*XP) is found in critical regulatory proteins including cdc25A, cdc25B, PKCgamma, IRS-1 and BCR (6). Although Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody binds 14-3-3 binding motif 2 with no requirement for proline in the +2 position, it provides a powerful tool for the discovery and characterization of potential 14-3-3 binding motif 2-containing proteins or other proteins with the RXY/FXS* motif. Specification REACTIVITY: All SENSITIVITY: Endogenous SOURCE: Rabbit