CST, 29943S, CD14 (61D3) Mouse Monoclonal Antibody (FITC Conjugate)

Monoclonal Antibody for studying CD14. Validated for Flow Cytometry (Fixed/Permeabilized),Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD14 (61D3) Mouse Monoclonal Antibody (FITC Conjugate) (CST #29943) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:20 Flow Cytometry (Live): 1:20 Storage Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity CD14 (61D3) Mouse Monoclonal Antibody (FITC Conjugate) recognizes endogenous levels of total CD14 protein. This antibody conjugate detects an epitope within the extracellular domain. Species Reactivity: Human Source / Purification This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Background CD14 is a leucine-rich repeat-containing pattern recognition receptor with expression largely restricted to the monocyte/macrophage cell lineage (1). Research studies have shown that CD14 is a bacterial lipopolysaccharide (LPS) binding glycoprotein, expressed as either a GPI-linked membrane protein or a soluble plasma protein (2). LPS induces an upregulation of GPI-linked CD14 expression, which facilitates TLR4 signaling and macrophage activation in response to bacterial infection (3-5). The 61D3 antibody is widely used to identify cells of the monocyte/macrophage lineage (6). Alternate Names CD14; CD14 antigen; CD14 molecule; Monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14, membrane-bound form; Monocyte differentiation antigen CD14, urinary form; Myeloid cell-specific leucine-rich glycoprotein Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1