CST, 3004S, Phospho-Thr-Pro-Glu (C32G12) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying . Validated for Western Blotting,Immunoprecipitation,Peptide ELISA (DELFIA). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Thr-Pro-Glu (C32G12) Rabbit Monoclonal Antibody (CST #3004) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Peptide ELISA (DELFIA): 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-Thr-Pro-Glu (C32G12) Rabbit Monoclonal Antibody detects endogenous levels of proteins containing phospho-threonine followed by proline and glutamine residues. This antibody also recognizes peptides and proteins containing the phospho-Thr-Pro motif. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide library containing the phospho-Thr-Pro-Glu motif. Background The phospho-Thr-Pro-Glu motif is a subgroup of the phospho-Thr-Pro motif. The Thr-Pro motif is phosphorylated by proline-directed protein kinases, such as MAP kinases and cyclin dependent kinases (CDKs). Proline-directed phosphorylation is one of the major regulatory phosphorylation events in many cellular processes, such as cell proliferation and differentiation (1-6). This phospho-Thr-Pro-Glu antibody provides a useful tool to study a subset of substrates of proline-directed kinases. Specification REACTIVITY: All SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG