CST, 30803S, ALDH1L1 (E7I2Q) Horse Chimeric Monoclonal Antibody

Monoclonal Antibody for studying ALDH1L1. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, ALDH1L1 (E7I2Q) Horse Chimeric Monoclonal Antibody (CST #30803) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity ALDH1L1 (E7I2Q) Horse Chimeric Monoclonal Antibody recognizes endogenous levels of ALDH1L1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification This recombinant chimeric antibody is engineered from ALDH1L1 (E7I2Q) Rabbit mAb #85828 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a horse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, ALDH1L1 (E7I2Q) Rabbit mAb #85828, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val431 of human ALDH1L1 protein. Background Aldehyde dehydrogenase 1 family member L1 (ALDH1L1), also known as 10-formyltetrahydrofolate dehydrogenase (FDH), is a member of the aldehyde dehydrogenase super family. It is a key enzyme in folate metabolism, playing an important role in regulation of cellular metabolism including proliferation (1,2). Downregulation of ALDH1L1 has been seen in tumors, leading to decreased suppression of cancer cell proliferation (2,3). ALDH1L1 has also been shown to be a useful astrocyte marker throughout the gray and white matter of the brain, labeling both the cell body and processes of astrocytes. ALDH1L1 does not label oligodendrocytes or neurons (4). It has been shown that expression of ALDH1L1 in astrocytes is stably expressed through normal tissue and during astrogliosis (5). Alternate Names 10-formyltetrahydrofolate dehydrogenase; 10-fTHF; 10-FTHFDH; AL1L1; aldehyde dehydrogenase 1 family member L1; aldehyde dehydrogenase 1 family, member L1; aldehyde dehydrogenase 1L1; Aldehyde dehydrogenase family 1 member L1; ALDH1L1; Cytosolic 10-formyltetrahydrofolate dehydrogenase; DKFZp781N0997; FDH; formyltetrahydrofolate dehydrogenase; FTHFD; MER57B-ALDH1L1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Horse chimeric IgG4