CST, 32904T, SARS-CoV-2 Membrane Protein (E3V5H) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Membrane Protein SARS-CoV-2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, SARS-CoV-2 Membrane Protein (E3V5H) Mouse Monoclonal Antibody (CST #32904) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SARS-CoV-2 Membrane Protein (E3V5H) Mouse Monoclonal Antibody recognizes endogenous levels of total SARS-CoV-2 membrane protein in infected cells. In some transfected models, a band of unknown origin, but not virus related, may be observed at approximately 50 kDa. Species Reactivity: Virus Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala183 of SARS-CoV-2 membrane protein. Background The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). Membrane protein is transcribed and translated from nested subgenomic RNA of the SARS-CoV-2 genome. Interaction of the membrane protein with the viral nucleocapsid protein is thought to stabilize the RNA-protein complex in the virions (3). Membrane protein has also been suggested to interact with the viral spike protein, but the functional consequence of that interaction is currently unclear (4). Alternate Names 2019-nCoV membrane protein; E1 glycoprotein; M protein; Matrix glycoprotein; membrane glycoprotein; Membrane Protein; SARS-CoV-2 membrane protein; severe acute respiratory syndrome coronavirus 2 membrane glycoprotein; VME1_SARS2 Specification REACTIVITY: Vir SENSITIVITY: Endogenous MW (kDa): 20-30 Source/Isotype: Mouse IgG3