CST, 33336S, SARS-CoV-2 Nucleocapsid Protein Antibody

Polyclonal Antibody for studying Nucleocapsid Protein SARS-CoV-2. Validated for Western Blotting. Highly specific and rigorously validated in-house, SARS-CoV-2 Nucleocapsid Protein Antibody (CST #33336) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SARS-CoV-2 Nucleocapsid Protein Antibody recognizes endogenous levels of total SARS-CoV-2 nucleocapsid (N) protein. Species Reactivity: Virus Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly30 of SARS-CoV-2 nucleocapsid (N) protein. Antibodies are purified by peptide affinity chromatography. Background The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). The nucleocapsid (N) protein is a phosphoprotein that plays a critical role in viral assembly (3,4). It binds to the viral genomic RNA, enabling its packaging into the ribonucleoprotein complex (RNP), and is the most abundant protein in the virion (5). Evidence from studies in SARS-CoV-2 and other coronaviruses suggest functions for nucleocapsid (N) protein beyond viral RNA packaging, including roles in viral replication (6) and suppressing host immune responses to viral infection (7). Alternate Names 2019-nCoV nucleocapsid protein; NC; NCAP_SARS2; nucleocapsid phosphoprotein; Nucleocapsid protein; Nucleoprotein; Protein N; SARS-CoV-2 nucleocapsid; Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2) Specification REACTIVITY: Vir SENSITIVITY: Endogenous MW (kDa): 55 SOURCE: Rabbit