CST, 33452S, C/EBP beta (E2K1U) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying C/EBP-beta. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, C/EBP beta (E2K1U) Rabbit Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #33452) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity C/EBP beta (E2K1U) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly67 of human C/EBPβ protein. Background C/EBPβ is a member of the transcription factor family of CCAAT/enhancer-binding proteins (C/EBPs) that are critical for cellular differentiation and function (1). There are various N-terminally truncated C/EBPβ isoforms: full-length C/EBPβ, C/EBPβ-LAP (liver-enriched activator protein), and C/EBPβ-LIP (liver-enriched inhibitory protein). In triple-negative breast cancer cells, aerobic glycolysis was shown to control the expression of C/EBPβ-LAP, which in turn stimulates the expression of granulocyte colony-stimulating factor (G-CSF) and granulocyte macrophage colony-stimulating factor (GM-CSF). These factors are secreted from the cancer cells to promote myeloid-derived suppressor cell (MDSC) development in the tumor microenvironment, thereby suppressing anti-tumor immunity (2). In addition, research studies showed that decreased C/EBPβ-LIP expression delays age-related phenotypes in mice, suggesting a potential role for C/EBPβ-LIP in aging (3). Alternate Names C/EBP beta; C/EBP-beta; CCAAT enhancer binding protein beta; CCAAT/enhancer binding protein (C/EBP), beta; CCAAT/enhancer-binding protein beta; CEBPB; CRP2; IL6DBP; interleukin 6-dependent DNA-binding protein; LAP; LIP; Liver activator protein; Liver-enriched inhibitory protein; liver-enriched transcriptional activator protein; MGC32080; NF-IL6; Nuclear factor NF-IL6; nuclear factor of interleukin 6; TCF-5; TCF5; Transcription factor 5; transcription factor C/EBP beta Specification REACTIVITY: H Mk SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG