CST, 33717T, SARS-CoV-2 Nucleocapsid Protein (E8R1L) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Nucleocapsid Protein SARS-CoV-2. Validated for WB,IHC,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, SARS-CoV-2 Nucleocapsid Protein (E8R1L) Mouse Monoclonal Antibody (CST #33717) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:800 - 1:3200 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity SARS-CoV-2 Nucleocapsid Protein (E8R1L) Mouse Monoclonal Antibody recognizes endogenous levels of total SARS-CoV-2 nucleocapsid (N) protein. This antibody does not cross-react with nucleocapsid proteins from SARS or MERS coronaviruses (assayed by western blot analysis). Species Reactivity: Virus Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly30 of SARS-CoV-2 nucleocapsid (N) protein. Background The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). The nucleocapsid (N) protein is a phosphoprotein that plays a critical role in viral assembly (3,4). It binds to the viral genomic RNA, enabling its packaging into the ribonucleoprotein complex (RNP), and is the most abundant protein in the virion (5). Evidence from studies in SARS-CoV-2 and other coronaviruses suggest functions for nucleocapsid (N) protein beyond viral RNA packaging, including roles in viral replication (6) and suppressing host immune responses to viral infection (7). Alternate Names 2019-nCoV nucleocapsid protein; NC; NCAP_SARS2; nucleocapsid phosphoprotein; Nucleocapsid protein; Nucleoprotein; Protein N; SARS-CoV-2 nucleocapsid; Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2) Specification REACTIVITY: Vir SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Mouse IgG2a