CST, 34140W2, Phospho-Stat4 (Tyr693) (D2E4) Rabbit Monoclonal Antibody (SignalFlex™ Alexa Fluor® 350 Conjugate)

Monoclonal Antibody for studying Stat4 (Tyr693) phosphate. Highly specific and rigorously validated in-house, Phospho-Stat4 (Tyr693) (D2E4) Rabbit Monoclonal Antibody (SignalFlex^™ Alexa Fluor^® 350 Conjugate) (CST #34140) is ready to ship. Product Usage Information SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate. Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Specificity / Sensitivity Phospho-Stat4 (Tyr693) (D2E4) Rabbit mAb (SignalFlex™ Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr693 of human Stat4 protein. Background The Jak-Stat signaling pathway is utilized by a large number of cytokines, growth factors, and hormones (1). Receptor-mediated tyrosine phosphorylation of Jak family members triggers phosphorylation of Stat proteins, resulting in their nuclear translocation, binding to specific DNA elements, and subsequent activation of transcription. The remarkable range and specificity of responses regulated by the Stats is determined, in part, by the tissue-specific expression of different cytokine receptors, Jaks, and Stats, as well as by the combinatorial coupling of various Stat members to different receptors (2). Stat4 is predominantly expressed in the spleen, thymus, and testis and has been most extensively investigated as the mediator of IL-12 responses (3-8). Activation of Stat4 is associated with phosphorylation at Tyr693 (9). Stat4 is activated in response to IL-2 in natural killer (NK) cells, but not in T cells (10). Alternate Names Signal transducer and activator of transcription 4; signal transducer and activator of transcription 4 variant 3; SLEB11; STAT4 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG