CST, 34256S, cGAS (E5V3W) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying cGAS. Validated for Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, cGAS (E5V3W) Rabbit Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #34256) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity cGAS (E5V3W) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn489 of human cGAS protein. Background Cyclic GMP-AMP synthase (cGAS, MB21D1) is an antiviral enzyme that produces the second messenger cyclic-GMP-AMP (cGAMP) in response to cytoplasmic DNA (1,2). The cGAS protein acts as a cytosolic DNA sensor that binds DNA and produces the cGAMP second messenger from ATP and GTP (1,2). cGAMP binds to and activates STING, a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1-4). STING is ubiquitously expressed and found predominantly in the ER (3). Following activation, STING translocates with TBK1 to perinuclear endosomes (5). The TBK1 kinase phosphorylates and activates interferon regulatory factors (IRFs) and NF-κB, which leads to the induction of type I interferon and other immune response genes (3-5). Alternate Names 2'3'-cGAMP synthase; C6orf150; CF150; cGAMP synthase; cGAS; Cyclic GMP-AMP synthase; FLJ39829; h-cGAS; LOC115004; Mab-21 domain containing 1; Mab-21 domain-containing protein 1; MB21D1; MGC131892; MGC142166; MGC142168; protein MB21D1 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG