CST, 34524S, Streptavidin (FITC Conjugate)

Avidin and Biotin for studying in the research area. Storage Store at 4°C. This product is stable for 12 months after receipt and is supplied in 10 mM HEPES, 0.15 M NaCl (pH 7.5), and 0.08% sodium azide. If further dilution of the conjugate is required, it is recommended to use a HEPES-, bicarbonate-, or phosphate-buffered saline solution. Protect from light. Avoid multiple freeze/thaw cycles . Specificity / Sensitivity Streptavidin has a remarkably high affinity for its natural ligand, biotin. The complex and irregular structure of the biotin-binding site makes it highly optimized for biotin binding and confers great specificity to the streptavidin-biotin complexes (4). Source / Purification Streptavidin is a protein that is derived from the bacteria . Streptavidin (FITC Conjugate) is purified by filtration. Background Streptavidin is a 53 kDa homotetramer isolated from for use in isolation and detection bioassays (3). Each streptavidin subunit forms high affinity non-covalent bonds with the vitamin biotin. Because of its strong non-covalent interaction with biotin, streptavidin can be used to isolate and detect biotinylated proteins (1,2).