CST, 34624S, CD31 (PECAM-1) (F2N3M) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying PECAM-1 mouse. Validated for IF,IF,F,F. Highly specific and rigorously validated in-house, CD31 (PECAM-1) (F2N3M) Rabbit Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #34624) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:100 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Flow Cytometry (Fixed/Permeabilized): 1:800 Flow Cytometry (Live): 1:800 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity CD31 (PECAM-1) (F2N3M) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse CD31 (PECAM-1) protein. Background CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members (2). The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules (3). Alternate Names C85791; Cd31; MGC102160; OTTMUSP00000003452; OTTMUSP00000003454; PECA1; Pecam; PECAM-1; PECAM-1/CD31; Pecam1; Platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule 1 Specification REACTIVITY: M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG