CST, 34859S, mNeonGreen-Tag (E2S6M) Mouse Monoclonal Antibody

Monoclonal Antibody for studying mNeonGreen lancelet. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, mNeonGreen-Tag (E2S6M) Mouse Monoclonal Antibody (CST #34859) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity mNeonGreen-Tag (E2S6M) Mouse Monoclonal Antibody detects recombinant proteins containing mNeonGreen protein. This antibody recognizes mNeonGreen protein fused to either the amino or carboxy terminus of targeted proteins in transfected cells. This antibody detects exogenously expressed mNeonGreen protein in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. This antibody does not cross-react with green-fluorescent protein (GFP). Non-specific signal of unknown origin may be observed in some rat cell lines. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu18 of mNeonGreen protein. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. mNeonGreen is a 220 amino acid, bright green-fluorescent protein derived from . mNeonGreen is a very useful tool as a fusion protein that reports gene expression, traces cell lineage, and defines subcellular protein localization (1). Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Mouse IgG1 kappa