CST, 35307S, G4S Linker (E7O2V) Mouse IgG2b Chimeric Monoclonal Antibody

Monoclonal Antibody for studying . Validated for Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, G4S Linker (E7O2V) Mouse IgG2b Chimeric Monoclonal Antibody (CST #35307) is ready to ship. Product Usage Information Flow Cytometry (Live): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Flow Cytometry (Live) Specificity / Sensitivity G4S Linker (E7O2V) Mouse IgG2b Chimeric Monoclonal Antibody recognizes exogenously expressed levels of scFv-based CARs containing a G4S linker. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing three Gly Ser repeats. The VH domain and VL domain of G4S Linker (E7O2V) Rabbit mAb are formatted into an scFv that is joined to the hinge and Fc domain of mouse IgG2b, resulting in a single-chain chimeric monoclonal antibody Background The poly-Glycine-Serine (G4S) linker is a type of flexible, unstructured synthetic peptide linker sequence often leveraged to connect the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The linker itself consists of a core pentapeptide sequence, Gly-Gly-Gly-Gly-Ser, that is repeated and commonly found as either a 15-mer (G4S) or 20-mer (G4S) within scFv-based CARs and scFv fragments. The linker sequence length plays a role in controlling scFv stability and the noncovalent association between the VH and VL domains (1,2). Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Mouse IgG2b