CST, 40843T, SARS-CoV-2 3C-Like Protease (E4D3W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying 3C-L proteinase SARS-CoV-2/3C-L proteinase SARS-CoV. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, SARS-CoV-2 3C-Like Protease (E4D3W) Rabbit Monoclonal Antibody (CST #40843) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SARS-CoV-2 3C-Like Protease (E4D3W) Rabbit Monoclonal Antibody recognizes endogenous levels of total SARS-CoV-2 3C-like protease protein. Species Reactivity: Virus Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys175 of SARS-CoV-2 3C-like protease protein. Background The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). SARS-CoV-2 3C-like protease, also known as Main protease (Mpro), is a non-structural protein (Nsp5) that is derived from ORF1a and ORF1b (2). In a sequence of processing events involving another viral protease, Nsp3 (papain-like protease), 3C-like protease is cleaved from pp1a and pp1ab polyproteins. 3C-like protease is a cysteine-like protease that importantly is responsible for all subsequent polyprotein cleavage events that give rise to the components of the viral replicase complex (3). For this reason, inhibitors of 3C-like protease are of interest as antiviral targets (3,4). Alternate Names 3C-like proteinase; 3C-like proteinase-pp1a-pp1ab; 3CL-PRO; 3CLp; 3CLpro; M pro; Mpro; nsp5; nsp5-pp1a/pp1ab (3CL-PRO); SARS-CoV 3C-like proteinase; SARS-CoV-2 3c-like proteinase Specification REACTIVITY: Vir SENSITIVITY: Endogenous MW (kDa): 38 Source/Isotype: Rabbit IgG