CST, 41859T, MERS-CoV Spike Protein (E6Q5A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Spike Protein MERS-CoV. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MERS-CoV Spike Protein (E6Q5A) Rabbit Monoclonal Antibody (CST #41859) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MERS-CoV Spike Protein (E6Q5A) Rabbit Monoclonal Antibody recognizes transfected levels of total MERS-CoV spike protein. This antibody detects full-length MERS-CoV spike protein and also detects the S1 fragment generated by host protease cleavage. This antibody does not cross-react with spike proteins from SARS coronaviruses (CoV-1, CoV-2). Species Reactivity: Virus Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human MERS-CoV spike protein. Background Middle East Respiratory Syndrome (MERS) is a contagious viral respiratory infection, whose causative agent was identified as the MERS-related coronavirus (MERS-CoV) (1,2). MERS-CoV is a single-stranded mRNA betacoronavirus; similar to other infectious coronaviruses (e.g., SARS-CoV-2), the MERS-CoV virion is comprised of four key structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (3). Coronavirus spike proteins are class I fusion proteins that harbor an ectodomain, a transmembrane domain, and an intracellular tail. The highly glycosylated ectodomain projects from the viral envelope surface and facilitates attachment and fusion with the host cell plasma membrane. The ectodomain can be further subdivided into host receptor-binding domain (RBD) (S1) and membrane-fusion (S2) subunits, which are produced upon proteolysis by host proteases (4). MERS-CoV spike proteins utilize the cell surface protein DPP4/CD26 as the receptor for cellular entry (5,6); notably, this protein displays no structural similarities to ACE2, the primary receptor for SARS-CoV-1 and SARS-CoV-2. Spike protein subunits represent a key antigenic feature of coronavirus virions, and therefore represent an important target of vaccines, novel therapeutic antibodies, and small-molecule inhibitors (7,8). Alternate Names E2; MERS Spike Glycoprotein; MERS-CoV spike protein; Peplomer protein; S glycoprotein; Spike glycoprotein; spike protein; SPIKE_CVEMC Specification REACTIVITY: Vir SENSITIVITY: Transfected Only MW (kDa): 200, 120 Source/Isotype: Rabbit IgG