CST, 4744S, CD15/SSEA1 (MC480) Mouse Monoclonal Antibody

Monoclonal Antibody for studying FUT4. Validated for Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized),Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, CD15/SSEA1 (MC480) Mouse Monoclonal Antibody (CST #4744) is ready to ship. Product Usage Information Immunofluorescence (Immunocytochemistry): 1:1600 - 1:3200 Flow Cytometry (Fixed/Permeabilized): 1:1600 Flow Cytometry (Live): 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 74180 . Protocol Available protocols: Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity CD15/SSEA1 (MC480) Mouse Monoclonal Antibody detects endogenous levels of SSEA1 antigen. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with F9 teratoma cells (X-irradiated). Background SSEA1 antibody detects a lactoseries oligosaccharide antigen that is expressed on the surface of mouse embryonal carcinoma and embryonic stem cells (1). This antigen is also found on early mouse embryos and both mouse and human germ cells, but is absent on human embryonic stem and carcinoma cells. Expression of SSEA1 in these human cell types increases upon differentiation, while on the mouse cell types differentiation leads to decreased expression (2). Alternate Names alpha (1,3) fucosyltransferase, myeloid-specific; Alpha-(1,3)-fucosyltransferase; Alpha-(1,3)-fucosyltransferase 4; CD15; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; ELFT; FCT3A; Fuc-TIV; Fucosyltransferase 4; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase IV; FucT-IV; FUT4; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; LeX; SSEA-1; stage-specific embryonic antigen 1 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): N/A Source/Isotype: Mouse IgM