CST, 4745S, TRA-1-81 (TRA-1-81) Mouse Monoclonal Antibody

Monoclonal Antibody for studying TRA-1-81 antigen. Validated for IHC,IF,F,F. Available in 2 sizes. Highly specific and rigorously validated in-house, TRA-1-81 (TRA-1-81) Mouse Monoclonal Antibody (CST #4745) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:100 Immunofluorescence (Immunocytochemistry): 1:1000 Flow Cytometry (Fixed/Permeabilized): 1:1600 Flow Cytometry (Live): 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity TRA-1-81 (TRA-1-81) Mouse Monoclonal Antibody detects endogenous levels of TRA-1-81 antigen. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with human embryonal carcinoma 2102Ep cl.2A6 cells. Background TRA-1-60 and TRA-1-81 antibodies detect antigens present on the surface of human stem, teratocarcinoma, and embryonic germ cells (1). TRA-1-60(S) reacts with a neuraminidase sensitive epitope of a proteoglycan (2,3), while TRA-1-81 reacts with a neuraminidase insensitive epitope on the same antigen. Recently this antigen has been proposed to be a form of the protein podocalyxin (4). TRA-1-60 is also detected in the serum of patients with germ cell tumors (5,6). Alternate Names GCTM-2 antigen; gp135; Gp200; MGC138240; PC; PCLP; PCLP-1; PCLP1; PDX; Podocalyxin; podocalyxin like; podocalyxin-like; Podocalyxin-like protein 1; PODXL; PODXL1; TRA-1-60 antigen; TRA-1-61 antigen; TRA-1-80 antigen; TRA-1-81 antigen Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 200-400 Source/Isotype: Mouse IgM