CST, 48780T, MAP2 (D5G1) Mouse Chimeric Monoclonal Antibody

Monoclonal Antibody for studying MAP2. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, MAP2 (D5G1) Mouse Chimeric Monoclonal Antibody (CST #48780) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity MAP2 (D5G1) Mouse Chimeric Monoclonal Antibody recognizes endogenous levels of total MAP2 protein. Species Reactivity: Mouse, Rat Source / Purification This recombinant chimeric antibody is engineered from MAP2 (D5G1) XP Rabbit mAb #8707 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a mouse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, MAP2 (D5G1) XP Rabbit mAb #8707, is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MAP2 protein. Background Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics, and organelle trafficking in axons and dendrites (1). Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B (280 and 270 kDa), and low molecular weight MAP2C and MAP2D (70 and 75 kDa). Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. GSK-3 and p44/42 MAP kinase phosphorylate MAP2 at Ser136, Thr1620, and Thr1623 (2,3). Phosphorylation at Thr1620/1623 by GSK-3 inhibits MAP2 association with microtubules and microtubule stability (3). Alternate Names DKFZp686I2148; MAP-2; MAP2; MAP2A; MAP2B; MAP2C; microtubule associated protein 2; Microtubule-associated protein 2; MTAP2 Specification REACTIVITY: M R SENSITIVITY: Endogenous Source/Isotype: Mouse chimeric IgG2a