CST, 49077SF, Rabbit (DA1E) Monoclonal Antibody IgG Isotype Control (BSA and Azide Free)

Monoclonal Antibody for studying . Validated for IHC,IF,F,F. Highly specific and rigorously validated in-house, Rabbit (DA1E) Monoclonal Antibody IgG Isotype Control (BSA and Azide Free) (CST #49077) is ready to ship. Product Usage Information This product is the carrier free version of product #3900. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 3900 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Rabbit (DA1E) Monoclonal Antibody IgG Isotype Control (BSA and Azide Free) is not directed against any known antigen. This antibody functions as an isotype control for rabbit IgG antibodies. Background Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to F receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody. Specification Concentration: 1 mg/ml SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG