CST, 50071S, PTMScan® HS Pilot Acetyl-Lysine Motif (Ac-K) Kit

PTMScan for studying in the research area. Storage All components in this kit are stable for at least 12 months when stored at the recommended temperature. Upon receipt, 99064P should be stored at 4°C. 25144P and 42424P should be stored at -20°C. Do not aliquot the antibody. Protocol Available protocols: PTMScan Background Acetylation of lysine, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (1). Signaling resulting in acetylation/deacetylation of histones, transcription factors, and other proteins affects a diverse array of cellular processes including chromatin structure and gene activity, cell growth, differentiation, and apoptosis (2-6). Recent proteomic surveys suggest that acetylation of lysine residues may be a widespread and important form of post-translational protein modification that affects thousands of proteins involved in control of cell cycle and metabolism, longevity, actin polymerization, and nuclear transport (7,8). The regulation of protein acetylation status is impaired in cancer and polyglutamine diseases (9), and HDACs have become promising targets for anti-cancer drugs currently in development (10).