CST, 52897SF, IL-6 (Siltuximab Biosimilar) Human Monoclonal Antibody

Monoclonal Antibody for studying IL6. Validated for Neutralizing. Highly specific and rigorously validated in-house, IL-6 (Siltuximab Biosimilar) Human Monoclonal Antibody (CST #52897) is ready to ship. Product Usage Information This product is intended for research use only (RUO). Optimal dilutions/concentrations should be determined by the end user. Formulation Supplied in 1X PBS, BSA and Azide Free. Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity IL-6 (Siltuximab Biosimilar) Human Monoclonal Antibody was confirmed to bind to its intended target protein IL-6 using bio-layer interferometry. Species Reactivity: Human Source / Purification IL-6 (Siltuximab Biosimilar) Human mAb is produced at Cell Signaling Technology. Siltuximabb is an antibody directed against human IL-6. Background Interleukin-6 (IL-6) is a pleiotropic cytokine with central roles in immune regulation, inflammation, hematopoiesis, and oncogenesis. Its biological activities are shared by other IL-6 family members, such as leukemia inhibitory factor and oncostatin M (1). To exert its biological effects, IL-6 must bind to the IL-6 receptor (IL-6R), composed of two alpha chains (IL-6Rα, 80 kDa) and two beta chains (IL-6Rβ or gp130, 130 kDa). Two moieties of IL-6 and two pairs of these receptor chains form a functional hexameric IL-6R complex. The subsequent intracellular signaling events are activated via gp130, which is the common signal transducer of cytokines related to IL-6. Activation of the IL-6R stimulates at least three major signaling pathways: JAK/STAT, PI-3 kinase, and MAPK signaling pathways (2,3). Alternate Names B cell stimulatory factor-2; B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; BSF2; CDF; CTL differentiation factor; HGF; HSF; Hybridoma growth factor; IFN-beta-2; IFNB2; IL-6; IL6; Interferon beta-2; interleukin 6; interleukin 6 (interferon, beta 2); interleukin BSF-2; Interleukin-6 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Human IgG1 kappa