CST, 55372S, Glucagon (F1A2G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Glucagon. Validated for Immunohistochemistry (Paraffin),Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Glucagon (F1A2G) Rabbit Monoclonal Antibody (CST #55372) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Frozen): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity Glucagon (F1A2G) Rabbit Monoclonal Antibody recognizes endogenous levels of total glucagon protein. Nonspecific staining was observed in neurites of the central and peripheral nervous systems by immunohistochemistry. This nonspecific signal is light and limited in human tissues and more prominent in rodent tissues. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr62 of human glucagon protein. Background Glucose homeostasis is regulated by a variety of hormones, including glucagon. Glucagon is synthesized as the precursor molecule proglucagon and is proteolytically processed to yield the mature peptide in α cells of the pancreatic islets. Glucagon causes the release of glucose from glycogen and stimulates gluconeogenesis in the liver (1,2). Alternate Names GCG; Glicentin; Glicentin-related polypeptide; GLP-1; GLP-1(7-36); GLP-1(7-37); GLP-2; GLP1; GLP2; GLUC; Glucagon; Glucagon-like peptide 1; Glucagon-like peptide 1(7-36); Glucagon-like peptide 1(7-37); Glucagon-like peptide 2; GRPP; Incretin hormone; OXM; OXY; Oxyntomodulin; preproglucagon; Pro-glucagon Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG