CST, 5605T, c-Myc (D84C12) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Myc. Validated for Western Blotting,Simple Western™,Immunofluorescence (Immunocytochemistry). Available in 3 sizes. Highly specific and rigorously validated in-house, c-Myc (D84C12) Rabbit Monoclonal Antibody (CST #5605) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 18927 . Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity c-Myc (D84C12) Rabbit Monoclonal Antibody detects endogenous levels of total c-Myc protein. This antibody is not recommended for detection of Myc-tagged fusion proteins (use Myc-Tag (9B11) Mouse Monoclonal Antibody#2276 or Myc-Tag (71D10) Rabbit Monoclonal Antibody #2278). Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced with a synthetic peptide corresponding to residues surrounding Asp30 of human c-Myc1 protein. Background Members of the Myc/Max/Mad network function as transcriptional regulators with roles in various aspects of cell behavior, including proliferation, differentiation, and apoptosis (1). These proteins share a common basic-helix-loop-helix leucine zipper (bHLH-ZIP) motif required for dimerization and DNA-binding. Max was originally discovered based on its ability to associate with c-Myc and found to be required for the ability of Myc to bind DNA and activate transcription (2). Subsequently, Max has been viewed as a central component of the transcriptional network, forming homodimers as well as heterodimers with other members of the Myc and Mad families (1). The association between Max and either Myc or Mad can have opposing effects on transcriptional regulation and cell behavior (1). The Mad family consists of four related proteins; Mad1, Mad2 (Mxi1), Mad3, and Mad4, and the more distantly related members of the bHLH-ZIP family, Mnt and Mga. Like Myc, the Mad proteins are tightly regulated with short half-lives. In general, Mad family members interfere with Myc-mediated processes, such as proliferation, transformation, and prevention of apoptosis by inhibiting transcription (3,4). Alternate Names avian myelocytomatosis viral oncogene homolog; BHLHE39; c-Myc; Class E basic helix-loop-helix protein 39; MRTL; MYC; Myc proto-oncogene protein; MYC proto-oncogene, bHLH transcription factor; myc-related translation/localization regulatory factor; MYCC; Proto-oncogene c-Myc; Transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog (avian) Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 57-65 Source/Isotype: Rabbit IgG