CST, 56263SF, BCL6 (E5I8I) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying BCL6. Validated for IHC Leica Bond,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, BCL6 (E5I8I) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #56263) is ready to ship. Product Usage Information This product is the carrier free version of product #89369. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 89369 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity BCL6 (E5I8I) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total BCL6 protein. This antibody is predicted to detect both known isoforms of human BCL6. Non-specific, non-nuclear staining was observed in human and mouse smooth muscle. Additional non-specific staining was observed in other mouse tissues. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro319 of human BCL6 protein. Background Chromosomal translocations result in misregulation of the proto-oncogene in patients with B cell-derived non-Hodgkin's lymphoma (1). The gene is selectively expressed in mature B cells and encodes a nuclear phosphoprotein that belongs to the BTB/POZ zinc finger family of transcription factors (2,3). BCL6 protein can bind to target DNA sequences of Stat6 and, analogous to Stat6, modulate the expression of interleukin-4-induced genes (4). Furthermore, BCL6 restrains p53-dependent senescence, making BCL6-active tumors functionally p53-negative (5). The mitogen-activated protein kinases, Erk1 and Erk2, but not JNK, phosphorylate BCL6 at multiple sites. Phosphorylation of BCL6 at Ser333 and Ser343 results in degradation of BCL6 by the ubiquitin/proteasome pathway in B cells (6,7). In addition, BCL6 is acetylated and its transcriptional repressor function is inhibited by the transcriptional co-activator p300 (8). Alternate Names B cell CLL/lymphoma 6; B-cell CLL/lymphoma 6; B-cell lymphoma 5 protein; B-cell lymphoma 6 protein; B-cell lymphoma 6 protein transcript; BCL-5; BCL-6; BCL5; BCL6; BCL6 transcription repressor; BCL6A; cys-his2 zinc finger transcription factor; LAZ3; lymphoma-associated zinc finger gene on chromosome 3; Protein LAZ-3; ZBTB27; Zinc finger and BTB domain-containing protein 27; Zinc finger protein 51; zinc finger transcription factor BCL6S; ZNF51 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG