CST, 57100T, Olig2 (E6G6Q) Horse Chimeric Monoclonal Antibody

Monoclonal Antibody for studying OLIG2. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Olig2 (E6G6Q) Horse Chimeric Monoclonal Antibody (CST #57100) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity Olig2 (E6G6Q) Horse Chimeric Monoclonal Antibody recognizes endogenous levels of total Olig2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification This recombinant chimeric antibody is engineered from Olig2 (E6G6Q) XP Rabbit mAb #65915 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a horse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, Olig2 (E6G6Q) XP Rabbit mAb #65915, is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human Olig2 protein. Background Olig2 is a basic helix-loop-helix (bHLH) transcription factor necessary for oligodendrocyte and motor neuron differentiation and development in the central nervous system (1,2). Olig2 has been shown to play a role in Purkinje cell Specification in the early cerebellum (3). Olig2 has also been shown to be a useful marker for primary and mature oligodendrocytes and is universally expressed in diffuse gliomas (4,5). Alternate Names basic domain, helix-loop-helix protein, class B, 1; BHLHB1; BHLHE19; Class B basic helix-loop-helix protein 1; Class E basic helix-loop-helix protein 19; human protein kinase C-binding protein RACK17; OLIG2; Oligo2; oligodendrocyte lineage transcription factor 2; Oligodendrocyte transcription factor 2; oligodendrocyte-specific bHLH transcription factor 2; PRKCBP2; protein kinase C binding protein 2; Protein kinase C-binding protein 2; Protein kinase C-binding protein RACK17; RACK17 Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Horse chimeric IgG4