CST, 57412S, T-bet/TBX21 (D6N8B) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3009)

Monoclonal Antibody for studying TBX21. Validated for Single Cell Analysis - InTraSeq™ 3'. Highly specific and rigorously validated in-house, T-bet/TBX21 (D6N8B) Rabbit Monoclonal Antibody (InTraSeq^™ 3' Conjugate 3009) (CST #57412) is ready to ship. Storage Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Single Cell Analysis - InTraSeq™ 3' Specificity / Sensitivity T-bet/TBX21 (D6N8B) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3009) recognizes endogenous levels of total T-bet/TBX21 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly465 of human T-bet/TBX21 protein. Background The gene family consists of transcription factors characterized by a related DNA-binding domain (T-box) of approximately 200 amino acids (1,2). The genes exhibit diverse temporal and spatial patterns in the developing embryo. Studies have demonstrated members of this family play crucial roles during embryogenesis in a wide range of organisms by regulating cell fate decisions to establish the early body plan and to regulate later processes underlying organogenesis (3-5). Mutations in genes are associated with many developmental defects (6). Recent studies also indicate potential roles in cancer by members of the T-box family (7-9). T-bet, also as known as TBX21, plays a critical role in development and maintenance of type 1 helper T (Th1) and T-bet deficient mice display impaired Th1 differentiation (10,11). Alternate Names T-bet; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor 21; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; T-PET; TBET; TBLYM; TBX21; Transcription factor TBLYM Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG