CST, 57446S, Neurofilament-L (C28E10) Mouse Chimeric Monoclonal Antibody

Monoclonal Antibody for studying NFL. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Neurofilament-L (C28E10) Mouse Chimeric Monoclonal Antibody (CST #57446) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity Neurofilament-L (C28E10) Mouse Chimeric Monoclonal Antibody recognizes endogenous levels of total Neurofilament-L protein. Species Reactivity: Human, Mouse, Rat Source / Purification This recombinant chimeric antibody is engineered from Neurofilament-L (C28E10) Rabbit mAb #2837 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a mouse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, Neurofilament-L (C28E10) Rabbit mAb #2837, is produced by immunizing animals with a synthetic peptide corresponding to Glu450 of human Neurofilament-L. Background The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Research studies have shown neurofilament accumulations in many human neurological disorders, including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1). Alternate Names 68 kDa neurofilament protein; CMT1F; CMT2E; CMTDIG; FLJ53642; light molecular weight neurofilament protein; NEFL; Neurofilament L; neurofilament light; Neurofilament light polypeptide; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament-light; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; NF-L; NF68; NFL; PPP1R110; protein phosphatase 1, regulatory subunit 110 Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Mouse chimeric IgG2a