CST, 57493S, SARS-CoV-2 RNA-dependent RNA Polymerase (E4X1Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RNA-dependent RNA polymerase-pp1ab SARS-CoV-2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, SARS-CoV-2 RNA-dependent RNA Polymerase (E4X1Z) Rabbit Monoclonal Antibody (CST #57493) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SARS-CoV-2 RNA-dependent RNA Polymerase (E4X1Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total SARS-CoV-2 RNA-dependent RNA polymerase protein in infected cells. In some lysates that were tested (non-Vero E6, non-infected), a faint, non-specific band around 110 kDa could be observed. Species Reactivity: Virus Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of SARS-CoV-2 RNA-dependent RNA polymerase protein. Background The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). SARS-CoV-2 RNA-dependent RNA polymerase, RdRp, is a non-structural protein (Nsp12) translated from ORF1b. It is the key enzyme essential for transcription of viral RNA from RNA templates and is therefore critical to replication of the virus (2). The polymerase has a structure similar to all viral RNA RdRps, the so-called hand structure: the catalytic module consists of palm, fingers, and thumb domains, forming the encircled human right hand model. Some conservation exists between different RNA viruses in the palm and finger domains, but there are significant differences in other domains reflecting different catalytic modes of action (3). SARS-CoV-2 RdRp requires accessory proteins, Nsp7 and Nsp8, along with Nsp14 for transcriptional activity (4). Alternate Names non-structural protein 12; nsp12; Nsp12 (4393-5324); Pol; RdRp; RNA-dependent RNA polymerase; RNA-dependent RNA polymerase-pp1ab; RNA-directed RNA polymerase; SARS-CoV-2 Nsp12 Specification REACTIVITY: Vir SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG