CST, 5757S, Phospho-Thr-Pro-Pro Motif [pTPP] (D61C3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying . Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Thr-Pro-Pro Motif [pTPP] (D61C3) Rabbit Monoclonal Antibody (CST #5757) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Thr-Pro-Pro Motif [pTPP] (D61C3) Rabbit Monoclonal Antibody recognizes endogenous levels of proteins only when phosphorylated within the TPP motif. This antibody does not cross react with phospho-serine or phospho-threonine in other contexts. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with synthetic peptide library containing the phospho-Thr-Pro-Pro motif. Background The phospho-Thr-Pro-Pro motif is a subgroup of the phospho-Thr-Pro motif that is phosphorylated by proline-directed protein kinases, including MAP kinases and cyclin dependent kinases (CDKs). Proline-directed phosphorylation is one of the major regulatory phosphorylation events in cell proliferation, cell differentiation, and a number of other essential cellular processes (1-6). This motif was identified in both phospho-proteomic and motif X analyses as a significant phospho-thr motif (7,8). Specification REACTIVITY: All SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG