CST, 59700T, Ki-67 (D3B5) Horse Chimeric Monoclonal Antibody

Monoclonal Antibody for studying KI-67. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Ki-67 (D3B5) Horse Chimeric Monoclonal Antibody (CST #59700) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity Ki-67 (D3B5) Horse Chimeric Monoclonal Antibody recognizes endogenous levels of total Ki-67 protein. Species Reactivity: Human, Mouse, Rat Source / Purification This recombinant chimeric antibody is engineered from Ki-67 (D3B5) Rabbit mAb #9129 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a horse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, Ki-67 (D3B5) Rabbit mAb #9129, is produced by immunizing animals with a synthetic peptide corresponding to a recombinant protein fragment corresponding to residues near the central region of human Ki-67 protein. Background Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival (3). Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases (4). Alternate Names antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Antigen Ki67; KI-67; KI67; KIA; marker of proliferation Ki-67; MIB-; MIB-1; MKI67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105 Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Horse chimeric IgG4