CST, 60143S, Phospho-Stat6 (Tyr641) (D8S9Y) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3101)

Monoclonal Antibody for studying Stat6 (Tyr641) phosphate. Validated for Single Cell Analysis - InTraSeq™ 3'. Highly specific and rigorously validated in-house, Phospho-Stat6 (Tyr641) (D8S9Y) Rabbit Monoclonal Antibody (InTraSeq^™ 3' Conjugate 3101) (CST #60143) is ready to ship. Storage Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Single Cell Analysis - InTraSeq™ 3' Specificity / Sensitivity Phospho-Stat6 (Tyr641) (D8S9Y) Rabbit mAb (InTraSeq™ 3' Conjugate 3101) recognizes endogenous levels of Stat6 protein only when phosphorylated at Tyr641. Weak cross-reactivity with other tyrosine-phosphorylated proteins has been observed in some cell lines. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr641 of human Stat6 protein. Background Upon activation by Janus kinases, Stat6 translocates to the nucleus where it regulates cytokine-induced gene expression. Stat6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13 (1-4). In addition, Stat6 is activated by IFN-α in B cells, where it forms transcriptionally active complexes with Stat2 and p48 (5,6). Protein phosphatase 2A is also involved in regulation of IL-4-mediated Stat6 signaling (7). Alternate Names D12S1644; IL-4 Stat; IL-4-STAT; Signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; STAT, interleukin4-induced; STAT6; STAT6B; STAT6C; transcription factor IL-4 STAT Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG