CST, 62771S, Phospho-YAP (Ser61) (E4Q8S) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3055)

Monoclonal Antibody for studying YAP (Ser61) phosphate. Validated for Single Cell Analysis - InTraSeq™ 3'. Highly specific and rigorously validated in-house, Phospho-YAP (Ser61) (E4Q8S) Rabbit Monoclonal Antibody (InTraSeq^™ 3' Conjugate 3055) (CST #62771) is ready to ship. Storage Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Single Cell Analysis - InTraSeq™ 3' Specificity / Sensitivity Phospho-YAP (Ser61) (E4Q8S) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3055) recognizes endogenous levels of YAP protein only when phosphorylated at Ser61. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser61 of human YAP protein. Background YAP (Yes-associated protein, YAP65) was first identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5). In its capacity as a transcriptional co-activator, YAP is now widely recognized as a central mediator of the Hippo Pathway, which plays a fundamental and widely conserved role in regulating tissue growth and organ size (6-8). Phosphorylation at multiple sites (e.g., Ser109, Ser127) by LATS kinases promotes YAP translocation from the nucleus to the cytoplasm, where it is sequestered through association with 14-3-3 proteins (7-9). These LATS-driven phosphorylation events serve to prime YAP for subsequent phosphorylation by CK1δ/ε in an adjacent phosphodegron, triggering proteasomal degradation of YAP (10). YAP function is influenced by cellular metabolic status. This was recognized after observing that AMPK, a master regulator of cell metabolism, directly phosphorylates YAP at multiple sites (e.g., Ser61, Ser94) during energy stress or nutritional deprivation (11-13). Phosphorylation of Ser61 by AMPK did not increase 14-3-3 binding or affect YAP subcellular localization, but nevertheless resulted in transcriptional repression of YAP target genes (12). Alternate Names 65 kDa Yes-associated protein; COB1; Protein yorkie homolog; Transcriptional coactivator YAP1; YAP; YAP1; YAP2; YAP65; Yes associated protein 1; Yes-associated protein 1; Yes-associated protein 1, 65kDa; yes-associated protein 2; Yes-associated protein YAP65 homolog; Yes1 associated transcriptional regulator; YKI; Yorkie homolog Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG