CST, 64370T, Parvalbumin (E8N2U) Horse Chimeric Monoclonal Antibody

Monoclonal Antibody for studying Parvalbumin mouse. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Parvalbumin (E8N2U) Horse Chimeric Monoclonal Antibody (CST #64370) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity Parvalbumin (E8N2U) Horse Chimeric Monoclonal Antibody recognizes endogenous levels of total parvalbumin protein. Species Reactivity: Human, Mouse, Rat Source / Purification This recombinant chimeric antibody is engineered from Parvalbumin (E8N2U) XP Rabbit mAb #80561 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a horse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, Parvalbumin (E8N2U) XP Rabbit mAb #80561, is produced by immunizing animals with a recombinant protein specific to the amino terminus of human parvalbumin protein. Background Parvalbumin (PV, PVALB) is a member of the EF-hand family of calcium-binding proteins, containing EF-hand domains with a high affinity for calcium and magnesium (1). Parvalbumin is expressed in both the soma and in processes of specific subclasses of GABAergic interneurons. Parvalbumin is a slow calcium buffer, meaning it accelerates the initial phase of decay of calcium after an action potential, which likely inhibits facilitation and is thought to regulate short-term synaptic plasticity (2,3). Altered function of PV positive interneurons has been implicated in many neurological diseases, such as Alzheimer's disease, autism spectrum disorder, schizophrenia, and bipolar disorder (4). Alternate Names Parv; parvalbumin; Parvalbumin alpha; PRVA; PV; Pva; Pvalb Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Horse chimeric IgG4