CST, 64989S, IRF-5 (E9I4Z) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3109)

Monoclonal Antibody for studying IRF-5. Validated for Single Cell Analysis - InTraSeq™ 3'. Highly specific and rigorously validated in-house, IRF-5 (E9I4Z) Rabbit Monoclonal Antibody (InTraSeq^™ 3' Conjugate 3109) (CST #64989) is ready to ship. Storage Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Single Cell Analysis - InTraSeq™ 3' Specificity / Sensitivity IRF-5 (E9I4Z) Rabbit mAb (InTraSeq™ 3' Conjugate 3109) recognizes human IRF-5 protein and is also reactive with mouse IRF-5 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human IRF-5 protein. Background Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2). Alternate Names Interferon regulatory factor 5; IRF-5; IRF5; SLEB10 Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG