CST, 65738T, HA-Tag (E3F8G) Mouse Monoclonal Antibody

Monoclonal Antibody for studying . Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, HA-Tag (E3F8G) Mouse Monoclonal Antibody (CST #65738) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity HA-Tag (E3F8G) Mouse Monoclonal Antibody detects recombinant proteins containing the HA epitope tag. This antibody recognizes the HA-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. This antibody detects a non-specific band of unknown origin in mouse cell line extracts at 70 kDa. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA). Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The HA-tag is derived from an epitope of the influenza hemagglutinin protein, which has been used extensively as a general epitope tag in expression vectors (1). Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Mouse IgG2a