CST, 68083S, HPC4-Tag Antibody

Polyclonal Antibody for studying . Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, HPC4-Tag Antibody (CST #68083) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity HPC4-Tag Antibody recognizes recombinant proteins containing the HPC4-tag. This antibody recognizes the HPC4-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. Species Reactivity: All Species Expected Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues of the HPC4-tag. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. HPC4-tag is a 12 amino acid sequence derived from protein C, which is a vitamin K-dependent plasma zymogen. Protein C is activated by proteolytic cleavage of the thrombin-thrombomodulin complex to form an anticoagulant enzyme (1,2). This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (3,4). Specification REACTIVITY: All SENSITIVITY: Transfected Only SOURCE: Rabbit