CST, 69341T, Cas9 (S. pyogenes) (E7M1H) Horse Chimeric Monoclonal Antibody

Monoclonal Antibody for studying Cas9 bacteria. Validated for Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Cas9 (S. pyogenes) (E7M1H) Horse Chimeric Monoclonal Antibody (CST #69341) is ready to ship. Product Usage Information Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Cas9 ( Species Reactivity: All Species Expected Source / Purification This recombinant chimeric antibody is engineered from Cas9 ( ) (E7M1H) XP Rabbit mAb #19526 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a horse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, Cas9 ( ) (E7M1H) XP Rabbit mAb #19526, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu833 of Cas9 ( ) protein. Background The CRISPR associated protein 9 (Cas9) is an RNA-guided DNA nuclease and part of the CRISPR antiviral immunity system that provides adaptive immunity against extrachromosomal genetic material (1). The CRISPR antiviral mechanism of action involves three steps: (i), acquisition of foreign DNA by host bacterium; (ii), synthesis and maturation of CRISPR RNA (crRNA) followed by the formation of RNA-Cas nuclease protein complexes; and (iii), target interference through recognition of foreign DNA by the complex and its cleavage by Cas nuclease activity (2). The type II CRISPR/Cas antiviral immunity system provides a powerful tool for precise genome editing and has potential for specific gene regulation and therapeutic applications (3). The Cas9 protein and a guide RNA consisting of a fusion between a crRNA and a trans-activating crRNA (tracrRNA) must be introduced or expressed in a cell. A 20-nucleotide sequence at the 5' end of the guide RNA directs Cas9 to a specific DNA target site. As a result, Cas9 can be "programmed" to cut various DNA sites both and in cells and organisms. CRISPR/Cas9 genome editing tools have been used in many organisms, including mouse and human cells (4,5). Research studies demonstrate that CRISPR can be used to generate mutant alleles or reporter genes in rodents and primate embryonic stem cells (6-8). Alternate Names cas9; CRISPR-associated endonuclease Cas9/Csn1; csn1 Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Horse chimeric IgG4