CST, 69486C, SARS-CoV-2 Spike RBD-ACE2 Blocking Antibody Detection ELISA Kit

ELISA Kit for studying Spike Protein SARS-CoV-2 in the research area. Protocol Available protocols: ELISA+ Specificity / Sensitivity The SARS-CoV-2 Spike RBD-ACE2 Blocking Antibody Detection ELISA Kit detects endogenous levels of antibodies that block the interaction between the SARS-CoV-2 spike RBD (318-541) protein and human ACE2 (18-615). This kit is designed to detect blocking antibodies present in human serum/plasma, however, it may also be used to assess blocking activity of non-human antibodies and small molecules. Species Reactivity: All Species Expected Background The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is similar to other coronaviruses, and is comprised of four key structural proteins: S, the spike protein, E, the envelope protein, M, the membrane protein, and N, the nucleocapsid protein (2). Coronavirus spike proteins are class I fusion proteins and harbor an ectodomain, a transmembrane domain, and an intracellular tail (3,4). The highly glycosylated ectodomain projects from the viral envelope surface and facilitates attachment and fusion with the host cell plasma membrane. The ectodomain can be further subdivided into host receptor-binding domain (RBD) (S1) and membrane-fusion (S2) subunits, which are produced upon proteolysis by host proteases at S1/S2 and S2' sites. S1 and S2 subunits remain associated after cleavage and assemble into crown-like homotrimers (2,4). In humans, both SARS-CoV and SARS-CoV-2 spike proteins utilize the angiotensin-converting enzyme 2 (ACE2) protein as a receptor for cellular entry (5-7). Spike protein subunits represent a key antigenic feature of coronavirus virions, and therefore represent an important target of vaccines, novel therapeutic antibodies, and small-molecule inhibitors (8,9). Alternate Names 2019-nCoV surface glycoprotein; Peplomer protein; S glycoprotein; SARS-CoV-2 spike protein; spike glycoprotein; SPIKE_SARS2; surface glycoprotein Specification REACTIVITY: All