CST, 69852S, Ezh2 (D2C9) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3081)

Monoclonal Antibody for studying EZH2. Validated for Single Cell Analysis - InTraSeq™ 3'. Highly specific and rigorously validated in-house, Ezh2 (D2C9) Rabbit Monoclonal Antibody (InTraSeq^™ 3' Conjugate 3081) (CST #69852) is ready to ship. Storage Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Single Cell Analysis - InTraSeq™ 3' Specificity / Sensitivity Ezh2 (D2C9) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3081) detects endogenous levels of total Ezh2 protein. This antibody conjugate does not cross-react with Ezh1 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg354 of human Ezh2 protein. Background The polycomb group (PcG) proteins are involved in maintaining the silenced state of several developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis (1,2). Enhancer of zeste homolog 2 (Ezh2), a member of this large protein family, contains four conserved regions, including domain I, domain II, and a cysteine-rich amino acid stretch that precedes the carboxy-terminal SET domain (3). The SET domain has been linked with histone methyltransferase (HMTase) activity. Moreover, mammalian Ezh2 is a member of a histone deacetylase complex that functions in gene silencing, acting at the level of chromatin structure (4). Ezh2 complexes methylate histone H3 at Lys9 and 27 , which is thought to be involved in targeting transcriptional regulators to specific loci (5). Ezh2 is deregulated in various tumor types, and its role, both as a primary effector and as a mediator of tumorigenesis, has become a subject of increased interest (6). Alternate Names enhancer of zeste 2; enhancer of zeste 2 polycomb repressive complex 2 subunit; Enhancer of zeste homolog 2; enhancer of zeste homolog 2 (Drosophila); ENX-1; ENX1; EZH1; EZH2; EZH2b; Histone-lysine N-methyltransferase EZH2; KMT6; KMT6A; Lysine N-methyltransferase 6; MGC9169; WVS; WVS2 Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG