CST, 7037V3, PathScan® Phospho-Chk2 (Thr68) Sandwich ELISA Kit

ELISA Kit for studying Chk2 (Thr68) phosphate in the research area. Protocol Available protocols: ELISA+ Specificity / Sensitivity PathScan Species Reactivity: Human Background Chk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50, and Thr68) each followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases (4,5). After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR (5-7). The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 at residues Thr383 and Thr387 in the activation loop of the kinase domain (8). Alternate Names Cds1; Cds1 homolog; Checkpoint kinase 2; checkpoint-like protein CHK2; CHEK2; CHK2; CHK2 checkpoint homolog; CHK2 checkpoint homolog (S. pombe); hCds1; HuCds1; LFS2; PP1425; protein kinase CHK2; RAD53; Serine/threonine-protein kinase Chk2 Specification REACTIVITY: H