CST, 7074S, Anti-rabbit IgG, HRP-linked Antibody

Secondary Antibody for studying in the research area. Product Usage Information Recommended Antibody Dilutions: 1:1000-1:3000 20X LumiGLO ® Reagent and 20X Peroxide #7003 1:1K-1:3K SignalFire™ ECL Reagent #6883 1:1K-1:3K SignalFire™ Plus ECL Reagent #12630 1:5K-1:15K SignalFire™ Elite ECL Reagent #12757 1:10K-1:20K Storage Supplied in 10mM sodium HEPES (pH 7.5), 150 mM NaCl, 2 mg/ml bovine serum albumin (BSA) and 50% glycerol. Store at -20°C. Do not aliquot the antibodies. This product is stable for 60 months when stored at -20C. Protocol Available protocols: Western Blotting Specificity / Sensitivity Species Reactivity: Rabbit Background Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times. Specification REACTIVITY: Rab Source/Isotype: Goat