CST, 71135T, CD27 (F3R2V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD27 mouse. Validated for IHC,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, CD27 (F3R2V) Rabbit Monoclonal Antibody (CST #71135) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:500 - 1:2000 Immunofluorescence (Frozen): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Flow Cytometry (Live): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Live) Specificity / Sensitivity CD27 (F3R2V) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD27 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of mouse CD27 protein. Background CD27 (TNFRSF7) is a transmembrane protein of the TNF receptor superfamily (TNFRSF). It is mainly expressed on lymphoid cells (also on early hematopoietic precursor cells in mice) (1,2). CD27 is considered a phenotypic marker for memory B cells and is also used to identify B regulatory (Breg) cells (3,4). It is constitutively expressed on naïve CD4 and CD8 T cells, and its expression is further upregulated upon T cell activation. CD27 is one of the two most important co-stimulatory receptors for T cell priming (the other one is CD28). While CD28 co-stimulatory signal mainly triggers cell proliferation, CD27 co-stimulatory signal primarily promotes cell survival and differentiation (5,6). Upon binding to its ligand CD70, CD27 activates the NF-κB and JNK signaling pathways through TNFR-associated factors (TRAFs), the adaptor molecules that are associated with CD27 cytoplasmic tail domain. Upon activation, CD27 is shed from the cell surface, and soluble CD27 is used as a marker of T cell activation (7,8). Alternate Names CD antigen 27; Cd27; CD27 antigen; CD27L receptor; OTTMUSP00000029268; OTTMUSP00000029269; S15; S152; T-cell activation antigen CD27; Tnfr; Tnfrsf7; Tp5; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG