CST, 7175C, PathScan® Phospho-MEK1 (Ser217/221) Sandwich ELISA Kit

ELISA Kit for studying MEK1 (Ser218/Ser222) phosphate in the research area. Protocol Available protocols: ELISA+ Specificity / Sensitivity PathScan Species Reactivity: Human, Mouse Background MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII. Alternate Names CFC3; Dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK 1; MEK1; MEL; mitogen-activated protein kinase kinase 1; MKK1; MP2K1; PRKMK1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) Specification REACTIVITY: H M