Proteintech, 66093-1-Ig, Caspase 8/P43/P18 Monoclonal antibody

Size: 20ul / 150ul The Caspase 8/P43/P18 (66093-1-Ig) by Proteintech is a Monoclonal antibody targeting Caspase 8/P43/P18 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 66093-1-Ig targets Caspase 8/P43/P18 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, HeLa cells, HEK-293 cells, HepG2 cells Positive IP detected in: HepG2 cells Positive IHC detected in: human liver cancer tissue, human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:100-1:400 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3 (PMID:21816816). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro- and cleaved-caspase 8. Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat, pig, monkey, chicken, sheep Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag20524 Product name: Recombinant human Caspase 8 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-270 aa of BC028223 Sequence: MDFSRNLYDIGEQLDSEDLASLKFLSLDYIPQRKQEPIKDALMLFQRLQEKRMLEESNLSFLKELLFRINRLDLLITYLNTRKEEMERELQTPGRAQISAYRVMLYQISEEVSRSELRSFKFLLQEEISKCKLDDDMNLLDIFIEMEKRVILGEGKLDILKRVCAQINKSLLKIINDYEEFSKERSSSLEGSPDEFSNGEELCGVMTISDSPREQDSESQTLDKVYQMKSKPRGYCLIINNHNFAKAREKVPKLHSIRDRNGTHLDAGTV Predict reactive species Full Name: caspase 8, apoptosis-related cysteine peptidase Calculated Molecular Weight: 538 aa, 62 kDa Observed Molecular Weight: 53-57 kDa, 32-45 kDa, 18 kDa GenBank Accession Number: BC028223 Gene Symbol: Caspase 8 Gene ID (NCBI): 841 RRID: AB_11232214 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q14790 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.