Proteintech, 83078-5-RR, p300 Recombinant monoclonal antibody

Size: 20ul / 100ul The p300 (83078-5-RR) by Proteintech is a Recombinant antibody targeting p300 in IF/ICC, FC (Intra), IP, ELISA, ChIP-qPCR applications with reactivity to human samples 83078-5-RR targets p300 in WB, IF/ICC, FC (Intra), IP, ELISA, ChIP-qPCR applications and shows reactivity with human samples. Tested Applications Positive IP detected in: HeLa cells Positive IF/ICC detected in: A431 cells, HepG2 cells Positive FC (Intra) detected in: THP-1 cells Positive ChIP-qPCR detected in: K-562 cells Recommended dilution Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information p300 is a 300 kDa global transcriptional coactivator with histone acetyltransferase (HAT) activity. p300 binds to chromosomal histones and modulates the chromatin conformation by acetylation of its histones, leading to activation of various gene transcriptions. p300 plays a critical role in DNA repair synthesis after DNA damage induced by ultraviolet or radiation. p300 is overexpressed in various types of cancer, which is associated with worse patient outcomes (PMID: 27322077). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: E1A binding protein p300 Calculated Molecular Weight: 264 kDa Observed Molecular Weight: 300 kDa GenBank Accession Number: NM_001429 Gene Symbol: EP300 Gene ID (NCBI): 2033 RRID: AB_3670795 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q09472 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.